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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in lots of tumors, which results in chromosome instability, and therefore may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a significant role in endorsing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two additional splice variants were being documented but in depth nucleotide sequence data isn't nonetheless out there.
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Blocking peptides are peptides that bind exclusively towards the focus on antibody and block antibody binding. These peptide typically has the epitope regarded because of the antibody.
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This manual outlines additional doable optimization of cellular and immunoassay parameters to be certain the very best outcomes are...
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This guideline outlines more probable optimization of cellular and immunoassay parameters to guarantee the best possible success are...
Antibodies certain to the blocking peptide not bind towards the epitope over the focus on protein. By comparing the staining with the blocked antibody vs . the antibody by itself, you can see which staining is specific.
Goal Data Cyclin E belongs to your remarkably conserved cyclin spouse and children, whose users are characterised by a dramatic periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins show unique expression and degradation styles which add to your temporal coordination of each and every mitotic celebration. Cyclin E varieties a fancy with and functions like a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates on the G1-S phase boundary and is degraded as cells development by S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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